Part:BBa_K4790046:Design

ASR1-Q79N-F

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 19
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 19
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 19
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 19
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 19
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Ⅰ The last base at the 3' end of the primer is selected as C or G.

Ⅱ Tm value of the primer should be controlled between 55℃ and 65℃.

Ⅲ The GC content of primers should be controlled between 40% and 60%.

Ⅳ Forward and reverse primer Tm values and GC content should be as consistent as possible.

Ⅴ The length of primer should be no more than 42bp generally.

Ⅵ △Tm should be limited to 2℃.

Source

It does not come from any genomic sequence but is designed according to node 59(BBa_K4790064).